Stacks 45431

Frol Frolbericheri (intersex woman) (main)
Vidmenir Knume (intersex man) (supporting)

Kaoru (non-binary?) (supporting)

Ushiyama (trans woman?) (supporting)

Creed Diskenth (gay?) (supporting)

Maria Oosawa (lesbian?) (protagonist)
Canaan (lesbian?) (main)
Liang Qi (lesbian) (supporting)

Dahlia (intersex woman) (supporting)
Ertegun (bi?) (supporting)
Cybelle (lesbian?) (supporting)
Desmond (bigender and intersex) (supporting)
Mermaid Sisters (agender) (minor)
Marie (bi) (minor)
Anne (lesbian) (minor)

Edward "Ed" Wong Hau Pepelu Tivrusky IV (genderqueer) (main)
Grencia "Gren" Mars Elijah Guo Eckener ("intersex" man and gay) (supporting)
Julius (gay) (minor)

Ikuno "Code:196" (lesbian) (main)
Nanami "Code 703" (lesbian) (supporting)
9'α (agender? and bi?) (supporting)

Lilis Joanca (trans woman) (minor)

Senkuu Ishigami (aroace) (protagonist)
Francois (non-binary?) (supporting)

Cocona Cocomine (lesbian?) (protagonist)
Papika (lesbian?) (main)

Peppo (trans woman) (supporting)

Rui Ninomiya (trans girl/gender non-conforming) (main)
O.D. (genderless) (main)
Berg Katze (genderless) (main)

Kyuubei Yagyuu (genderqueer?) (supporting)
Haji (non-binary?) (supporting)
Azumi "Agomi" Agonoske (gay and drag queen) (supporting)
Tokumori Saigou (drag queen) (supporting)
Hachirou (trans man and gay) (supporting)
Honjou Kyoushirou (gay) (supporting)
Shimura Tae (bi) (supporting)

Daishikyou/Archbishop (agender and intersex) (supporting)

Hasuta (gay) (main)

Berg Katse (intersex and genderfluid?) (main)

Luca Esposito (intersex boy and bi) (main)

Osamu (gay) (protagonist)
Kimera (intersex man and gay) (main)
Kianu (gay) (supporting)

Kino (agender) (protagonist)

Motoko Kusanagi (bi) (protagonist)

Mikihisa (trans man) (supporting)

Nanachi (non-binary?) (main)

Rin Asogi (bi) (protagonist)
Mimi (lesbian) (main)
(Name removed for spoilers) (agender and intersex) (main)
Female informants (lesbians) (minor)
Mary (trans woman) (minor)
Sue (drag queen) (minor)

Criff Cay (trans woman) (supporting)

Kite (trans man) (supporting)

Christina Matsuzaki (trans woman?) (supporting)

Shion (gay) (protagonist)
Nezumi (gay) (main)
Inukashi/Dogkeeper (non-binary) (supporting)

Yayoi Kunizuka (lesbian) (supporting)
Shion Karanomori (bi) (supporting)
Rikako Ouryou (lesbian) (supporting)
Rina Takazaki (lesbian) (minor)

Yuuta Iridatsu (trans boy?, gender-bending/body swap) (protagonist)

Nenene Sumiregawa (lesbian?) (protagonist)
Yomiko Readman (lesbian?) (main)
Anita (lesbian?) (main)
Nancy Makuhari (lesbian?) (supporting)
Hisami Hishiishi (lesbian) (supporting)

Momoko (trans woman) (supporting)
Miiko Kumagai (trans woman) (supporting)

Shinji Ikari (bi) (protagonist)
Kaworu Nagisa (gay or pan) (supporting)

Saki Watanabe (bi/pan) (protagonist)
Satoru Asahina (bi/pan) (main)
Maria Akizuki (bi/pan) (main)
Shun Aonuma (bi/pan) (main)

Izana Shinatose (third gender/intersex girl) (main)

Ruka "Rukako" Urushibara (trans woman) (supporting)

Rackage/Lukkage (bi) (supporting)

Haruhi Suzumiya (bi) (main)

Leeron "Ron" Littner (gay) (supporting)

Kiruko (trans woman?) (protagonist)

Nathan Seymour/Fire Emblem (agender and bi) (supporting)

Kuroko Shirai (bi) (supporting)

Elendira the Crimsonnail (trans woman) (supporting)

Kuroumaru Tokisaka (genderless) (main)

Ranban (intersex man?) (supporting)

Inga (genderfluid?, gender-bending) (main)